A MODIFIED COTTON-WOOL COLUMN METHOD FOR THE RAPID RECOVERY OF DNA FROM AGAROSE GEL SLICE
โ Scribed by BAOYI LIU; WEICHEN XU; ZHIYAO LUO; QIUYUN LIU
- Book ID
- 111344572
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 125 KB
- Volume
- 16
- Category
- Article
- ISSN
- 1060-3999
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๐ SIMILAR VOLUMES
A procedure for quick and simple elution of DNA from agarose gels is presented. After electrophoresis, bands of interest are cut out of the gel and the slices are equilibrated in a neutral salt buffer. The slices are then frozen and centrifuged through a filtration assembly whereby the DNA-containin
## Abstract A simple elution tube has been constructed which allows reliable electroelution of DNA restriction fragments from agarose and acrylamide gels. This method enables the recovery of DNA fragments of 50 to over 50 000 base pairs from cylindrical or slab gels with yields between 70 % to 90 %