After electrophoresis, DNA can be efficiently recovered by solubilization of agarose gels with NaC104, followed by retention of DNA on glass fiber filters. After removal of the NaCIO, by ethanol, the DNA can be extracted with a low salt buffer.
Recovery of DNA from agarose gel with home-made silica milk
โ Scribed by Wang Zhuo-hua; Li Hong-hao; Ma Hui-wen
- Publisher
- Wuhan University
- Year
- 2000
- Tongue
- English
- Weight
- 337 KB
- Volume
- 5
- Category
- Article
- ISSN
- 1007-1202
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
A simple and reliable method for the recovery of specific fragments of DNA from agarose gels is presented. The electroelution of the DNA onto the NENSORB cartridge matrix with the subsequent elution of the bound DNA by a methanol (50% v/v) wash has been shown to result in the quantitative recovery o
A procedure for quick and simple elution of DNA from agarose gels is presented. After electrophoresis, bands of interest are cut out of the gel and the slices are equilibrated in a neutral salt buffer. The slices are then frozen and centrifuged through a filtration assembly whereby the DNA-containin