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Rapid and simple isolation of DNA from agarose gels

✍ Scribed by Martin Grey; Martin Brendel

Publisher: Springer-Verlag
Year: 1992
Tongue: English
Weight: 152 KB
Volume: 22
Category: Article
ISSN: 0172-8083
DOI: 10.1007/bf00351746

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✦ Synopsis

We present a simple method for the isolation of DNA from agarose gels that is economic, fast, and independent of electrical equipment. DNA fragments of up to 6 kb can be easily extracted within 5 min using a disposable plastic syringe and filter paper. Total extraction of DNA fragments between 10 and 20 kb in size is achieved by concentrating the DNA flushed from the gel in a DNA-binding column.

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