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Preparation of thymidine and deoxyuridine labelled with 14C in base

✍ Scribed by Jiři Filip; Zdeněkňejedlÿ

Publisher: John Wiley and Sons
Year: 1967
Tongue: French
Weight: 421 KB
Volume: 3
Category: Article
ISSN: 0022-2135
DOI: 10.1002/jlcr.2590030208

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✦ Synopsis

Deoxyribosides of thymine and uracil labelled with 14C of specife activity identical with that of the original radioactive base were prepared by single-step enzymatic synthesis. When employing 2-deoxyy-cr,-D-riboso-I-phosphate of dicyclohexylamine salt, the enzymatic preparation isolated .from Escherichia coli B gives satisfactory yields in the synthesis of both deoxyribonucleosides. Studies were made oj the time course of reactions, as well as of the effects of some components on the yields. It has been found that the enzymatic preparation from Escherichia coli B facilitates the transfer of the deoxyribosylase group between thymine and thymidine. The employed technique separation allows preparation of radioactive uracil and thymine deoxyribosides of a radiochemical purity exceeding 99 per cent.

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