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Facile and economical preparation of [14C]-labelled shikimic acid

✍ Scribed by Annette Krüper; Peter Gehrke; Nikolaus Amrhein

Publisher
John Wiley and Sons
Year
1990
Tongue
French
Weight
224 KB
Volume
28
Category
Article
ISSN
0022-2135

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✦ Synopsis

Abstract

A convenient and inexpensive method for the preparation of [^14^C]‐labelled shikimic acid based on the photoassimilation of ^14^CO~2~ by henbane (Hyoscyamus niger L.) leaves in the presence of the herbicide glyphosate is described.

Methanolic extracts were purified by successive anion exchange, paper and thin‐layer chromatography to yield [^14^C]‐labelled shikimic acid of 99.5% radiochemical purity, as shown by analytical HPLC. Under the conditions employed, the rate of incorporation of ^14^CO~2~ into shikimic acid (0.7 – 17.6%) showed a positive correlation with the size of the leaf used in the incubation (3.6 ‐ 146 mg fresh weight), while the specific activity of the acid (6 ‐ 12.7 GBq/mmol) was an inverse function of the leaf size.

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