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Micro-scale preparation of 4-thiouracil labelled with 14C or 14C + 35S

โœ Scribed by J. Seda; L. I. Votruba; R. Tykva

Publisher
John Wiley and Sons
Year
1974
Tongue
French
Weight
308 KB
Volume
10
Category
Article
ISSN
0022-2135

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โœฆ Synopsis

Abstract

A selective and quantitative method was developed for a microscale preparation of 4โ€thiouracilโ€2โ€^14^C by the direct thiation of uracilโ€2โ€^14^C. From pyridine, dioxane, and tetralin as the thiation solvents, the best yield was obtained in dioxane (96.6% of 4โ€thiouracilโ€2โ€^14^C). The simultaneous labelling with ^14^C + ^35^S was obtained by isotope exchange with elemental sulfurโ€^35^S on refluxing in dimethylformamide. The present procedure may be used as a general method for the preparation of ^14^C or ^14^C + ^35^S labelled bases of nucleic acids of both the pyrimidine and purine type as well as the corresponding nucleosides and nucleotides.

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