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Preparation of L-cysteine-35S hydrochloride by reduction of L-cystine-35s

✍ Scribed by M. B. Skakun-Todorovi; S. R. Albahari

Publisher
John Wiley and Sons
Year
1979
Tongue
French
Weight
201 KB
Volume
16
Category
Article
ISSN
0022-2135

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✦ Synopsis

The method for the synthesis of L-cysteine hydrochloride labelled with 35S is described. L -C ~s t i n e -~~S obtained from baker's yeast was reduced with tin in hydrochloric acid and radiochemically pure L-cysteine-35S hydrochloride was isolated by ion-exchange chromatography on a column.

The obtained L -~y s t e i n e -~~S hydrochloride was stable in aqueous solution, pH 2, for six months and had specific activity of 20-125 mCi/mmol and radiochemical purity better than 9 5 % .

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