The method for the synthesis of L-cysteine hydrochloride labelled with 35S is described. L -C ~s t i n e -~~S obtained from baker's yeast was reduced with tin in hydrochloric acid and radiochemically pure L-cysteine-35S hydrochloride was isolated by ion-exchange chromatography on a column. The obta
Facile synthesis of D,L-[34S]cysteine hydrochloride
โ Scribed by Tuong Huynh-Ba; Laurent Fay
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- French
- Weight
- 128 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0022-2135
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โฆ Synopsis
Abstract
The title compound was synthesized at the gram level in 66% yield by addition of [^34^S]thioacetic acid to ฮฑโacetamidoacrylic acid, followed by acid hydrolysis.
๐ SIMILAR VOLUMES
L-[ 35S]Homocysteine thiolactone has been synthesized by demethylation of L-{ 35S]Methionine with sodium in liquid ammonia and subsequent lactonisation in acid solution. The radiochemical yield of the carrier added synthesis was in the range of 45 to 50 % with a radiochemical purity higher than 96 %
tremely wide range of M r (for example, 10 -200 kDa using 10% polyacrylamide gel) using the single-gel electrophoretic system. In conclusion, laborious work and time for the electrophoresis can be shortened using the single-gel system.