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Preparation of 35S-labelled l-methionine and l-cystine by biosynthesis

✍ Scribed by S. R. Albahari; M. B. Skakun-Todorovi

Publisher
John Wiley and Sons
Year
1978
Tongue
French
Weight
495 KB
Volume
14
Category
Article
ISSN
0022-2135

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✦ Synopsis

Abstract

The modified method for biosynthesis of L‐methionine‐^35^S and L‐cystine‐^35^S from baker's yeast (Saccharomyces cerevisiae) is described. The yeast was cultivated in a sulphur‐deplated medium containing carrier‐free Na~2~^35^SO~4~, under air bubbling during 24 hours. Yeast proteins were hydrolyzed enzymatically into free amino acids, and L‐methionine‐^35^S and L‐cystine‐^35^S were isolated by ion‐exchange chromatography on a column. Radioactive yields for L‐methionine‐^35^S and L‐cystine‐^35^S were 30% and 10%, respectively. The obtained products have high specific activity, 1–10 Ci/mmol, and radiochemical purity better than 95%.

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