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Preparation of high-specific-activity d-[3-3H]pantothenic acid

✍ Scribed by David S. Vallari; Charles O. Rock

Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
411 KB
Volume
154
Category
Article
ISSN
0003-2697

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✦ Synopsis

High-specific-activity D-[3-3H]pantothenic acid (5 Ci/mmol) was prepared from commercially available beta-[3-3H]alanine employing Escherichia coli strain DV1 (panD2 pan F1). This strain is defective in beta-alanine synthesis and pantothenate uptake, and under appropriate growth conditions converted 85 to 90% of the input beta-[3-3H]alanine to extracellular D-[3-3H]pantothenate. The radiolabeled vitamin was purified from the medium by thin-layer chromatography followed by reverse-phase high-performance liquid chromatography. The overall yield of D-[3-3H]pantothenic acid was 30% and radiochemical purity was greater than 99%.

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