✦ LIBER ✦
Preparation of UDP-d-[U-14C]galacturonic acid and UDP-d-[6-3H]galactose with high specific activities
✍ Scribed by A.Kalyan Rao; Joseph Mendicino
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 447 KB
- Volume
- 72
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
Simple procedures for the preparation of UDP-D-[U-'*C]galBCtUrOniC acid and UDP-D-[6+i]gatactose with high specific activities are described. The conversion involves the enzymatic oxidation of the appropriate UDP-o-galactose derivative to UDP-o-galactose-6aldehyde
followed by either further oxidation with iodine or reduction with sodium borotritide. The labeled products were isolated from reaction mixtures by ion exchange and paper chromatography.