A sensitive tluorometric assay for DNA determination using m-diaminobenzoic acid dihydrochloride for the reaction with deoxyribose liberated by perchloric acid is described. Fluorescence is proportional to the amount of DNA over the range 0.05-5 pg of DNA when the reaction is conducted in a volume o
Optimal conditions for the fluorometric determination of DNA
β Scribed by Forbes W. Robertson; Kirsteen Tait
- Publisher
- Elsevier Science
- Year
- 1971
- Tongue
- English
- Weight
- 245 KB
- Volume
- 41
- Category
- Article
- ISSN
- 0003-2697
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The diaminobenzoic acid dihydrochloride fluorescence method has been examined to determine optimal conditions for the assay of DNA in perchloric acid extracts and in pretreated Chlamydomonas cells on glass-fiber filters. Maximal fluorescence yield was obtained by allowing: (a) the perchloric acid ex
An interesting observation reported in our previous studies (1,2) is that, on binding to nucleic acids (NA) the dye, ethidium bromide (EB) increases its fluorescence quantum efficiency by a factor of 20 to 25. In consequence of the EB absorption spectrum shift on binding, for a suitably chosen wavel
## 210 nization. This method may prove useful for other tis-rates of three competing conformational reactions that occur when thermally denatured, complementary ccl sues as well. and non-ccl ss DNAs are rapidly cooled. When thermally denatured non-ccl DNA is rapidly cooled, the REFERENCES strands
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