𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Optimal conditions for the fluorometric determination of DNA

✍ Scribed by Forbes W. Robertson; Kirsteen Tait


Publisher
Elsevier Science
Year
1971
Tongue
English
Weight
245 KB
Volume
41
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.


πŸ“œ SIMILAR VOLUMES


A sensitive fluorometric assay for the d
✍ Richard VytΓ‘Ε‘ek πŸ“‚ Article πŸ“… 1982 πŸ› Elsevier Science 🌐 English βš– 421 KB

A sensitive tluorometric assay for DNA determination using m-diaminobenzoic acid dihydrochloride for the reaction with deoxyribose liberated by perchloric acid is described. Fluorescence is proportional to the amount of DNA over the range 0.05-5 pg of DNA when the reaction is conducted in a volume o

Fluorometric determination of DNA in Chl
✍ Torleiv Lien; Gjert Knutsen πŸ“‚ Article πŸ“… 1976 πŸ› Elsevier Science 🌐 English βš– 410 KB

The diaminobenzoic acid dihydrochloride fluorescence method has been examined to determine optimal conditions for the assay of DNA in perchloric acid extracts and in pretreated Chlamydomonas cells on glass-fiber filters. Maximal fluorescence yield was obtained by allowing: (a) the perchloric acid ex

A new fluorometric method for RNA and DN
✍ Jean-Bernard Le Pecq; Claude Paoletti πŸ“‚ Article πŸ“… 1966 πŸ› Elsevier Science 🌐 English βš– 487 KB

An interesting observation reported in our previous studies (1,2) is that, on binding to nucleic acids (NA) the dye, ethidium bromide (EB) increases its fluorescence quantum efficiency by a factor of 20 to 25. In consequence of the EB absorption spectrum shift on binding, for a suitably chosen wavel

Fluorometric Assay for the Determination
✍ P.G. Penketh; K. Shyam; A.C. Sartorelli πŸ“‚ Article πŸ“… 1997 πŸ› Elsevier Science 🌐 English βš– 82 KB

## 210 nization. This method may prove useful for other tis-rates of three competing conformational reactions that occur when thermally denatured, complementary ccl sues as well. and non-ccl ss DNAs are rapidly cooled. When thermally denatured non-ccl DNA is rapidly cooled, the REFERENCES strands

Fluorometric determination of DNA in fix
✍ Knut-Jan Andersen; Dankert W. Skagen πŸ“‚ Article πŸ“… 1977 πŸ› Elsevier Science 🌐 English βš– 359 KB

The measurement of DNA in tissue samples fixed in ethanol/acetic acid is described. Small, fixed tissue samples are digested by warm alkaline treatment followed by neutralization with HCl, and DNA is determined by complex formation with the dye ethidium bromide (EB). When standard DNA from calf thym