✦ LIBER ✦

Fluorometric determination of DNA in Chlamydomonas

✍ Scribed by Torleiv Lien; Gjert Knutsen

Publisher: Elsevier Science
Year: 1976
Tongue: English
Weight: 410 KB
Volume: 74
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(76)90238-4

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✦ Synopsis

The diaminobenzoic acid dihydrochloride fluorescence method has been examined to determine optimal conditions for the assay of DNA in perchloric acid extracts and in pretreated Chlamydomonas cells on glass-fiber filters. Maximal fluorescence yield was obtained by allowing: (a) the perchloric acid extract to react with 4-6% diaminobenzoic acid dihydrochloride for 20-40 min at 60-80°C at about pH 3.0; (b) the pretreated cells on glass-fiber filters to react with a 20% solution of diaminobenzoic acid dihydrochloride for 40 min at 60°C. METHODS AND MATERIALS 1. Reagents. (a) DABA *2HCf was prepared from 3,Sdiaminobenzoic acid according to the method of Holm-Hansen et al. (4). By using this 560 Copyright

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