𝔖 Bobbio Scriptorium
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A sensitive fluorometric assay for the determination of DNA

✍ Scribed by Richard Vytášek

Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
421 KB
Volume
120
Category
Article
ISSN
0003-2697

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✦ Synopsis

A sensitive tluorometric assay for DNA determination using m-diaminobenzoic acid dihydrochloride for the reaction with deoxyribose liberated by perchloric acid is described. Fluorescence is proportional to the amount of DNA over the range 0.05-5 pg of DNA when the reaction is conducted in a volume of 400 ~1 and over the range 0.01-0.5 pg when the reaction is conducted in a volume of 40 ~1. The assay is suitable for estimating DNA both in perchloric acid extracts and in complex mixtures with protein and RNA. The determination of DNA in cells grown as a monolayer by the described method is simple and rapid.

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