✦ LIBER ✦
Fluorometric Assay for the Determination of DNA–DNA Cross-Links Utilizing Hoechst 33258 at Neutral pH Values
✍ Scribed by P.G. Penketh; K. Shyam; A.C. Sartorelli
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 82 KB
- Volume
- 252
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
210
nization. This method may prove useful for other tis-rates of three competing conformational reactions that occur when thermally denatured, complementary ccl sues as well.
and non-ccl ss DNAs are rapidly cooled. When thermally denatured non-ccl DNA is rapidly cooled, the REFERENCES strands undergo a rapid intramolecular mispairing re-1. Macdonald, G. J., Demers, L. M., and Greep, R. O. (1973) Fertil. action before any significant proportion of the mole-Steril. 24, 98-103.
cules begins to anneal. Annealing involves a slow asso-