Fluctuation analyses of spontaneous mutations to 6-thioguanine resistance in Chinese hamster ovary cells in culture
β Scribed by J. Patrick O'Neill; Patricia A. Brimer; Abraham W. Hsie
- Book ID
- 113192102
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 619 KB
- Volume
- 82
- Category
- Article
- ISSN
- 0027-5107
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The temporal sequence of mutation to 6-thioguanine resistance was studied in Chinese hamster ovary cells synchronised in S phase. The number of resistant mutants induced following exposure to pulses of bromodeoxyuridine and subsequent selection was significantly greater if BUdR pulses were administe
## Abstract The mutation rate to thioguanine resistance was 3.11 Γ 10^β6^ in a near diploid V79 hamster cell line and 7.58 Γ 10^β8^ in a near tetraploid derivative produced with colchicine. The specific activities of glucoseβ6βphosphate dehydrogenase and phosphoglycerate kinase of the tetraploid li