Spontaneous mutation rate to thioguanine resistance is decreased in polyploid hamster cells
✍ Scribed by John Morrow; Douglas Stocco; Emily Barron
- Book ID
- 102310279
- Publisher
- John Wiley and Sons
- Year
- 1978
- Tongue
- English
- Weight
- 358 KB
- Volume
- 96
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
The mutation rate to thioguanine resistance was 3.11 × 10^−6^ in a near diploid V79 hamster cell line and 7.58 × 10^−8^ in a near tetraploid derivative produced with colchicine. The specific activities of glucose‐6‐phosphate dehydrogenase and phosphoglycerate kinase of the tetraploid line were greater than that of the diploid which suggests that twice the number of active X chromosomes were present in the tetraploid. These results are compatible with the hypothesis that spontaneous variants resistant to thioguanine arise through mutation and chromosomal segregation, as has been suggested for induced mutations in tetraploid hamster cells.