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Temporal sequence of mutation for 6-thioguanine resistance in synchronised Chinese hamster cells

✍ Scribed by I. Raskó; K. Burg; L. Dallmann

Book ID
104791680
Publisher
Springer
Year
1976
Tongue
English
Weight
656 KB
Volume
48
Category
Article
ISSN
0040-5752

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✦ Synopsis

The temporal sequence of mutation to 6-thioguanine resistance was studied in Chinese hamster ovary cells synchronised in S phase. The number of resistant mutants induced following exposure to pulses of bromodeoxyuridine and subsequent selection was significantly greater if BUdR pulses were administered during the first, rather than the second half of S phase. The results are interpreted as indicating that the gene responsible for 6-thioguanine resistance is replicated during the first half of S phase.

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A series of mitochondrially inherited chloramphenicol-resistant (CAP-R) mutants were isolated in Chinese hamster cells. To determine whether the Chinese hamster CAP-R mutations were homologous to those isolated in mouse and human cell culture systems, we determined the nucleotide sequence of the reg