A new cell line derived from a woodchuck hepatocellular carcinoma serially transplanted in athymic nude mice has been established and named WH257GE10. The original tumor in the nude mouse system produces woodchuck hepatitis surface antigen and albumin. In addition, woodchuck hepatitis virus DNA is i
Establishment and Characterization of a Woodchuck Hepatocellular Carcinoma Cell Line (WH44KA)
โ Scribed by Abe, Kenji ;Kurata, Takeshi ;Yamada, Ken-ichiro ;Okumura, Hideo ;Shikata, Toshio
- Book ID
- 118699764
- Publisher
- Wiley (Blackwell Publishing)
- Year
- 1988
- Tongue
- English
- Weight
- 859 KB
- Volume
- 79
- Category
- Article
- ISSN
- 0910-5050
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A new cell line derived from a woodchuck hepatitis surface antigen-positive woodchuck hepatocellular carcinoma has been established and named T3-HEP-W 1. This new cell line was established directly from a primary woodchuck hepatocellular carcinoma. Adaptation of the cells to the in vitro culture con
The normal woodchuck karyotype was determined by the G-banding technique, and the chromosomal abnormalities of a woodchuck hepatocellular carcinoma-derived cell line (WH257GE10) were analyzed and compared with it. The normal woodchuck karyotype was 38,XY in a male, and 38,XX in a female. Autosomes w
Woodchucks free from woodchuck hepatitis virus were treated with diethylnitrosamine in vivo for 2 months, and then hepatocytes obtained by enzymatic perfusion were cultured with the hepatopromoter phenobarbital. This in vivo-in vitro procedure gave rise to proliferating epithelial cell foci, from on
The chromosomal sites of woodchuck hepatitis virus (WHV) DNA integration were identified in a woodchuck hepatocellular carcinoma-derived cell line (WH257GE 10) by the in situ hybridization technique using 'H-labelled WHV whole genome (WHV 2) as a probe. The C-banded chromosome spreads from WH257GE 1