Analysis of the chromosomal abnormalities of a woodchuck hepatocellular carcinoma-derived cell line (WH257GE10)

✍ Scribed by Fumiaki Matsushita; Shinsuke Ohno; Masashi Unoura; Kenichi Kobayashi; Nobuyoshi Tanaka; Kenichi Fukuoka; Takeshi Morioka; Shuichi Kaneko; Syou Aoyama; Shunsuke Migita; Nobu Hattori

• Publisher: John Wiley and Sons
• Year: 1987
• Tongue: French
• Weight: 932 KB
• Volume: 40
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910400524

✦ Synopsis

The normal woodchuck karyotype was determined by the G-banding technique, and the chromosomal abnormalities of a woodchuck hepatocellular carcinoma-derived cell line (WH257GE10) were analyzed and compared with it. The normal woodchuck karyotype was 38,XY in a male, and 38,XX in a female. Autosomes were classified into 2 groups (A and B). Group A consisted of 13 pairs (1-13) of metacentric or submetacentric chromosomes, and group B consisted of 5 pairs (14-18) of telocentric or sub-telocentric ones. The X chromosome was sub-metacentric, and the Y chromosome was telocentric. In WH257GE10, the modal number was 36. Deletion of one of the chromosome 5 pair, of the chromosome 13 pair, and of the Y Chromosome was observed. Two marker chromosomes (MI and M3 were found. These markers showed the same G-banded patterns except for a small chromosome fragment at the distal part of the long arm of MI. The chromosome aberrations were very stable through successive passages in vitro. These findings suggest that the chromosomal abnormalities found in WH257GE10 might be associated with the pathogenesis of woodchuck hepatocellular carcinoma.