Chromosomal assignment of woodchuck hepatitis virus (WHV) dna integration sites in a woodchuck hepatocellular carcinoma-derived cell line (WH257GE10)

✍ Scribed by Yasutsugu Mizuno; Seishi Murakami; Fumiaki Matsushita; Masashi Unoura; Ken-Ichi Kobayashi; Shunsuke Migita; Nobu Hattori; Sinsuke Ohno

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: French
• Weight: 713 KB
• Volume: 43
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910430419

✦ Synopsis

The chromosomal sites of woodchuck hepatitis virus (WHV) DNA integration were identified in a woodchuck hepatocellular carcinoma-derived cell line (WH257GE 10) by the in situ hybridization technique using 'H-labelled WHV whole genome (WHV 2) as a probe. The C-banded chromosome spreads from WH257GE 10 were identified and diagrammed schematically according to their band patterns. WHV DNA was integrated into 2 sites: 33 region of the long arm of chromosome 6 (6q33) and 31 region of the long arm of chromosome 8 (8q31). Chromosomal sites of WHV DNA integration were stable during successive passage periods analyzed in vitro.