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Establishment of a new cell line from a woodchuck hepatocellular carcinoma

✍ Scribed by Shin Ohnishi; Hiromu Aoyama; Junji Shiga; Yuji Itai; Takashi Moriyama; Takashi Ishikawa; Nobuo Sasaki; Koshi Yamamoto; Kaoru Koshimizu; Shuichi Kaneko; Seishi Murakami; Nobu Hattori; Michio Imawari

Publisher: John Wiley and Sons
Year: 1988
Tongue: English
Weight: 510 KB
Volume: 8
Category: Article
ISSN: 0270-9139
DOI: 10.1002/hep.1840080121

No coin nor oath required. For personal study only.

✦ Synopsis

A new cell line derived from a woodchuck hepatitis surface antigen-positive woodchuck hepatocellular carcinoma has been established and named T3-HEP-W 1. This new cell line was established directly from a primary woodchuck hepatocellular carcinoma. Adaptation of the cells to the in vitro culture condition was completed after 3 months, with the doubling time of 24 hr. The morphologic features of the cell by light microscopy were of an epithelial type. The modal chromosome number was 100. Ornithine and tyrosine aminotransferase activities were detected. Production of albumin was negative. Integration of woodchuck hepatitis virus DNA was demonstrated by Southern blot analysis, although the secretion of woodchuck hepatitis surface antigen was not detected. T3-HEP-W1 is quite different from the previously reported WH257GE10 cell line and provides another in vitro model for the study of human hepatocellular carcinoma related to hepatitis B virus.

Woodchuck hepatitis virus (WHV), one of the hepadna viruses (l), is closely related to woodchuck hepatocellular carcinoma (HCC). HCC usually develops in animals positive for woodchuck hepatitis surface antigen (WHsAg) (2). In contrast, development of HCC is very rare in WHsAg-negative animals. WHV and HCC of woodchuck have been regarded as a suitable model for investigation of the genesis of human HCC related to hepatitis B virus. Woodchuck HCC cell line, WH257GE10, derived from a transplanted tumor in a nude mouse, has been established and reported by Unoura et al. (3). This paper reports the establishment and characterization of a new cell line of woodchuck HCC derived directly from a primary woodchuck HCC.

Methods

A male woodchuck (Marmota monax) positive for WHsAg was purcha5ed from Hazleton, Dutchland (Denver, Tumor.

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