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Hepatocellular carcinoma after thorotrast exposure: Establishment of a new cell line (Mz-Hep-1)

✍ Scribed by Wolfgang G. Dippold; Hans-Peter Dienes; Alexander Knuth; Walter Sachsse; Winfried Prellwitz; Dieter Bitter-Suermann; Karl-Hermann Meyer zum Büschenfelde

Publisher: John Wiley and Sons
Year: 1985
Tongue: English
Weight: 856 KB
Volume: 5
Category: Article
ISSN: 0270-9139
DOI: 10.1002/hep.1840050609

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✦ Synopsis

A human hepatoma cell line, associated with thorotrast exposure, from an hepatitis B markernegative patient was established as a permanent cell line (Mz-Hep-1) in tissue culture. Histology of the primary tumor, as well as phase contrast, transmission and scanning electron microscopy of the cultured cells showed typical characteristics of liver cells. Mz-Hep-1 cells secreted complement components (C2, C3, C4), carcinoembryonic antigen, lactate dehydrogenase, chymotrypsin, haptoglobin and retinol-binding protein and expressed HLA-, transferrin-, blood group B-related determinants and complement component C5 and carcinoembryonic antigen on their cell surface. Mz-Hep-1 cells represent the first human hepatoma cell line, which is strongly associated with a carcinogen.

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