Growth control and gene expression in a new hepatocellular carcinoma cell line, Hep40: Inhibitory actions of vitamin K
✍ Scribed by Boumediene Bouzahzah; Yuji Nishikawa; Dr. Daniela Simon; Brian I. Carr
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 938 KB
- Volume
- 165
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
Abstract
The growth characteristics of a newly established cell line, Hep40, derived from a human hepatoma are described. An absolute requirement was found for serum to mediate cell growth. Neither EGF, TGF‐α, nor HGF altered cell growth in the presence or absence of serum. A partial suppression of cell growth was achieved by several TGF‐β family proteins. Affinity crosslinking gels using ^125^I‐labeled TGF‐β showed a significant decrease in the TGF‐β cell‐surface type II receptor in Hep40 cells, compared to the TGF‐β‐sensitive Hep3B cell line. However, growth could be completely suppressed by addition of vitamins K to the culture medium in both Hep40 and several other hepatoma cell lines. Growth suppression by vitamins K was accompanied by an increased level of transcripts for c‐myc, c‐jun, and prothrombin genes, in contrast to the actions of TGF‐β1 protein, which caused a decrease in the level of c‐myc transcripts. These data show that this new human hepatoma cell line has partial resistance to growth inhibition by TGF‐β with a unique TGF‐β receptor defect. However, growth was completely suppressed by vitamins K. The differing gene expression patterns in response to TGF‐β as compared to vitamin K suggest that these two growth inhibitors act through differing pathways. © 1995 Wiley‐Liss Inc.