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Characterization of cell lines established from human hepatocellular carcinoma

✍ Scribed by Jae-Gahb Park; Jae-Ho Lee; Myung-Soo Kang; Kyu-Joo Park; You-Me Jeon; Hyun-Ju Lee; Hae-Sung Kwon; Hyun-Sook Park; Kyong-Sook Yeo; Kuhn-Uk Lee; Soo-Tae Kim; June-Key Chung; You-Jin Hwang; Hyo-Suk Lee; Chung Yong Kim; Young Ik Lee; Tchaw-Ren Chen; Robert J. Hay; Sang-Yong Song; Woo-Ho Kim; Chul-Woo Kim; Yong-Il Kim

Publisher: John Wiley and Sons
Year: 1995
Tongue: French
Weight: 975 KB
Volume: 62
Category: Article
ISSN: 0020-7136
DOI: 10.1002/ijc.2910620308

No coin nor oath required. For personal study only.

✦ Synopsis

We characterized 8 human hepatocellular-carcinoma cell lines established from the primary tumors of Korean patients. All lines showed substrate adherence and one line from anaplastic tumor also grew as floating aggregates. Most cultured cells maintained many morphological characteristics of the original tumors from which they were derived. Doubling times varied from 34 to 72 hr. All lines showed relatively high viability and were not contaminated with Mycoplasma or bacteria. All lines showed aneuploidy and were proven to be unique by D N A fingerprinting analysis. Hepatitis-B-virus (HBV) D N A was integrated in the genomes of all lines. Two of the cell lines (SNU-354, SNU-368) showed expression of HBV and HBVx (HBx) transcripts. SNU-354 strongly expressed albumin, and SNU-368 expressed transferrin and insulin-like growth factor II. No lines produced alpha-fetoprotein at the RNA and protein level. These cell lines represent useful tools for in vitro studies related to hepatocellular carcinoma.

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