Persistence of hepatitis C virus RNA in established human hepatocellular carcinoma cell lines

✍ Scribed by Tsuboi, So; Nagamori, Seishi; Miyazaki, Masahiro; Mihara, Koichiro; Fukaya, Kenichi; Teruya, Kiichiro; Kosaka, Tsunenori; Tsuji, Takao; Namba, Masayoshi

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 975 KB
• Volume: 48
• Category: Article
• ISSN: 0146-6615
• DOI: 10.1002/(sici)1096-9071(199602)48:2<133::aid-jmv3>3.0.co;2-a

✦ Synopsis

The persistence of the viral RNA of hepatitis C virus (HCV) was examined in 13 hepatocellular carcinoma (HCC) and two hepatoblastoma cell lines by reverse transcription followed by the polymerase chain reaction (RT-PCR). HCV RNA was detected in three HCC lines (JHH-1, JHH-4, and JHH-6) and negative-strand viral RNA was found in JHH-4, indicating that there is a putative replicative intermediate of HCV in JHH-4 cells. To rule out the possibility of contamination, the partial nucleotide sequences of HCV-specific PCR products of these three cell lines were determined. The clone from JHH-1 belonged to genotype 1 ( l a or 1 b), and the clones from JHH-4 and JHH-6 belonged to genotype 2b, but their sequences differed from each other. These cell lines may be useful for studies related t o HCV.