✦ LIBER ✦

Establishment and characterization of a diethylnitrosamine-initiated woodchuck hepatocyte cell line

✍ Scribed by Gang-Hong Lee; Tokuichi Kawaguchi; Kimie Nomura; Tomoyuki Kitagawa

Publisher: John Wiley and Sons
Year: 1987
Tongue: English
Weight: 601 KB
Volume: 7
Category: Article
ISSN: 0270-9139
DOI: 10.1002/hep.1840070524

No coin nor oath required. For personal study only.

✦ Synopsis

Woodchucks free from woodchuck hepatitis virus were treated with diethylnitrosamine in vivo for 2 months, and then hepatocytes obtained by enzymatic perfusion were cultured with the hepatopromoter phenobarbital. This in vivo-in vitro procedure gave rise to proliferating epithelial cell foci, from one of which the presently described hepatocyte cell line (WLC-3) was established and characterized.

WLC-3 cells possess morphological and biochemical features of differentiated hepatocy$es, including glucose-6-phosphatase activity and albumin production. Histopathological analysis of the tumor which developed transitorily in nude mouse subcutis after inoculation of the cell line revealed glandular structures comprising cells of hepatocellular-like morphology.

This is the first established woodchuck hepatocyte cell line free from woodchuck hepatitis virus and is therefore expected to be useful for studying the mode of gene expression and viral proliferation of woodchuck hepatitis virus and the mechanisms underlying woodchuck hepatitis virus-related hepatocarcinogenesis.

Woodchuck (Marmota monux) is the natural host of the woodchuck hepatitis virus (WHV) which belongs to hepadnaviridae together with human hepatitis B virus (1). Like human hepatitis B virus carriers, WHV carrier animals are at very high risk of developing chronic hepatitis and hepatocellular carcinomas (HCCs) (2). The

📜 SIMILAR VOLUMES

Establishment of a cell line from a wood

Establishment of a cell line from a woodchuck hepatocellular carcinoma

✍ Masashi Unoura; Kenichi Kobayashi; Kenichi Fukuoka; Fumiaki Matsushita; Hideo Mo 📂 Article 📅 1985 🏛 John Wiley and Sons 🌐 English ⚖ 703 KB

A new cell line derived from a woodchuck hepatocellular carcinoma serially transplanted in athymic nude mice has been established and named WH257GE10. The original tumor in the nude mouse system produces woodchuck hepatitis surface antigen and albumin. In addition, woodchuck hepatitis virus DNA is i

Establishment of a new cell line from a

Establishment of a new cell line from a woodchuck hepatocellular carcinoma

✍ Shin Ohnishi; Hiromu Aoyama; Junji Shiga; Yuji Itai; Takashi Moriyama; Takashi I 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 510 KB

A new cell line derived from a woodchuck hepatitis surface antigen-positive woodchuck hepatocellular carcinoma has been established and named T3-HEP-W 1. This new cell line was established directly from a primary woodchuck hepatocellular carcinoma. Adaptation of the cells to the in vitro culture con

Establishment and characterization of a

Establishment and characterization of a human neuroblastoma cell line

✍ Susanna Scarpa; Carlo Dominici; Paola Grammatico; Giuseppe Del Porto; Giuseppe R 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 French ⚖ 919 KB

A continuous human cell line RN-GA was established from a stage-Ill primary neuroblastoma prior to therapy. Light and electron microscopic analysis of the biopsy showed morphological features typical of neuroectodermal origin. Relative cellular DNA content and N-myc oncogene copy number were also an

Establishment and characterization of an

Establishment and characterization of an uveal-melanoma cell line

✍ Itte De Waard-Siebinga; Derk-Jan R. Blom; Marieke Griffioen; Peter I. Schrier; E 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 French ⚖ 996 KB

## Abstract A human uveal melanoma cell line (92–1) was established from a primary uveal melanoma, and has now been maintained in culture for over 2½ years. Light microscopy of the cultured cells demonstrated extremely pleiomorphic cells with large prominent nucleoli. Cell proliferation was determi

Establishment and characterization of a

Establishment and characterization of a human myeloma cell line (KMM-1)

✍ Atsushi Togawa; Nobumasa Inoue; Huminori Hyodo; Masayoshi Namba; Kanji Miyamoto 📂 Article 📅 1982 🏛 John Wiley and Sons 🌐 French ⚖ 821 KB

## Abstract A new cell line (KMM‐1) was established from a subcutaneous plasmacytoma of a 62‐year‐old male with multiple myeloma. Immunological studies indicated that cultured cells were derived from the same clone of myeloma cells __in vivo__: smeared cells were stained with fluoresceinconjugated

Establishment and characterization of hu

Establishment and characterization of human gastric carcinoma cell lines

✍ Jae-Gahb Park; Han-Kwang Yang; Woo Ho Kim; June-Key Chung; Myung-Soo Kang; Jae-H 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 French

We report 8 newly established gastric-carcinoma cell lines (SNU-216, 484, 520, 601, 620, 638, 668, 719) from Korean patients. Morphologic study was carried out using light and electron microscopes. CEA, aFP, and CA 19-9 and TPA in supernatant and in cell lysate were measured by radioimmunoassay. p53