✦ LIBER ✦

Establishment and characterization of a human myeloma cell line (KMM-1)

✍ Scribed by Atsushi Togawa; Nobumasa Inoue; Huminori Hyodo; Masayoshi Namba; Kanji Miyamoto

Publisher: John Wiley and Sons
Year: 1982
Tongue: French
Weight: 821 KB
Volume: 29
Category: Article
ISSN: 0020-7136
DOI: 10.1002/ijc.2910290502

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

A new cell line (KMM‐1) was established from a subcutaneous plasmacytoma of a 62‐year‐old male with multiple myeloma. Immunological studies indicated that cultured cells were derived from the same clone of myeloma cells in vivo: smeared cells were stained with fluoresceinconjugated globulin of antisera monospecific to lambdachain, and lambda‐chains in the cell extracts and in culture media were identical to the Bence‐Jones protein found in the patient's urine. The cell line grew in suspension with a doubling time of 36‐40 h and contained primitive plasmablastoid cells with prominent nucleoli and rough endoplasmic reticulum. Cells had the karyotype of 47, X, ‐Y. lq+, ‐2, +t(1:2) (cen:cen), +7, 12q+, 14q+, +mar and carried no Epstein‐Barr virus‐determined nuclear antigen. Surface markers were as follows; E rosette (‐), IgG Fc receptor (‐), C3 receptor (‐), S‐lg (+), TdT (‐), asialo‐GM, (‐). The reasons for the successful establishment of the myeloma cell line are discussed.

📜 SIMILAR VOLUMES

Establishment and characterization of a

Establishment and characterization of a human neuroblastoma cell line

✍ Susanna Scarpa; Carlo Dominici; Paola Grammatico; Giuseppe Del Porto; Giuseppe R 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 French ⚖ 919 KB

A continuous human cell line RN-GA was established from a stage-Ill primary neuroblastoma prior to therapy. Light and electron microscopic analysis of the biopsy showed morphological features typical of neuroectodermal origin. Relative cellular DNA content and N-myc oncogene copy number were also an

Establishment and characterization of a

Establishment and characterization of a human acute monocytic leukemia cell line (THP-1)

✍ Shigeru Tsuchiya; Michiko Yamabe; Yoshiko Yamaguchi; Yasuko Kobayashi; Tasuke Ko 📂 Article 📅 1980 🏛 John Wiley and Sons 🌐 French ⚖ 523 KB

## Abstract A human leukemic cell line (THP‐1) cultured from the blood of a boy with acute monocytic leukemia is described. This cell line had Fc and C3b receptors, but no surface or cytoplasmic immunoglobulins. HLA haplotypes of THP‐1 were HLA‐A2, ‐A9, ‐B5, ‐DRW1 and ‐DRW2. The monocytic nature of

IgD myeloma presenting as a testicular t

IgD myeloma presenting as a testicular tumor: Establishment and characterization of an IgD-secreting myeloma cell line

✍ Kazuyoshi Ishii; Kenji Yamato; Ichiro Kubonishi; Hirokuni Taguchi; Yuji Ohtsuki; 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 838 KB

A 72-year-old man presented with a left testicular tumor and underwent orchiectomy. The tumor was massively infiltrated with myeloma cells bearing monoclonal cytoplasmic IgDX. Three months after orchiectomy, he developed huge abdominal masses and subsequently ascites containing numerous myeloma cell

Establishment and characterization of hu

Establishment and characterization of human gastric carcinoma cell lines

✍ Jae-Gahb Park; Han-Kwang Yang; Woo Ho Kim; June-Key Chung; Myung-Soo Kang; Jae-H 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 French

We report 8 newly established gastric-carcinoma cell lines (SNU-216, 484, 520, 601, 620, 638, 668, 719) from Korean patients. Morphologic study was carried out using light and electron microscopes. CEA, aFP, and CA 19-9 and TPA in supernatant and in cell lysate were measured by radioimmunoassay. p53

Establishment of a human medulloblastoma

Establishment of a human medulloblastoma cell line

✍ R. M. McAllister; H. Isaacs; R. Rongey; M. Peer; W. Au; S. W. Soukup; M. B. Gard 📂 Article 📅 1977 🏛 John Wiley and Sons 🌐 French ⚖ 840 KB

## Abstract A cell line consisting of polygonal and fusiform cells was derived from a cerebellar medulloblastoma. Cells grew to high population density in liquid medium and formed colonies in agar medium. No neural or glial elements could be demonstrated in the cultured cells by electron microscopy

Genetic and biological characterization

Genetic and biological characterization of human myeloma cell lines: An overwiew of the lines established at Kawasaki Medical School

✍ Takemi Otsuki; Osamu Yamada; Kenichiro Yata; Naozo Nakazawa; Masafumi Taniwaki; 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 503 KB