We characterized 8 human hepatocellular-carcinoma cell lines established from the primary tumors of Korean patients. All lines showed substrate adherence and one line from anaplastic tumor also grew as floating aggregates. Most cultured cells maintained many morphological characteristics of the orig
Establishment and characterization of human metastatic hepatocellular carcinoma cell line
β Scribed by Jian-Ming Wen; Jie-Fu Huang; Liang Hu; Wei-Sheng Wang; Meng Zhang; Jonathan S.T. Sham; Jian-Min Xu; Wei-Fen Zeng; Dan Xie; Li-Jian Liang; Xin-Yuan Guan
- Book ID
- 114135291
- Publisher
- Elsevier Science
- Year
- 2002
- Tongue
- English
- Weight
- 297 KB
- Volume
- 135
- Category
- Article
- ISSN
- 0165-4608
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We report 8 newly established gastric-carcinoma cell lines (SNU-216, 484, 520, 601, 620, 638, 668, 719) from Korean patients. Morphologic study was carried out using light and electron microscopes. CEA, aFP, and CA 19-9 and TPA in supernatant and in cell lysate were measured by radioimmunoassay. p53
## Abstract A continuous human cell line was established from a hepatocellular carcinoma of an HBsAgβpositive Japanese male. The cell line, designated HCCβM, grows as an adhering monolayer with a doubling time of 24 h in medium RPMIβ1640 supplemented with 10% FCS and grows with 30% clonal efficienc