✦ LIBER ✦

Enzymatic synthesis of [11C]N-acetylserotonin

✍ Scribed by Geert Mannens; Guido Slegers; Patrick Goethals

Publisher: John Wiley and Sons
Year: 1990
Tongue: French
Weight: 294 KB
Volume: 28
Category: Article
ISSN: 0022-2135
DOI: 10.1002/jlcr.2580280308

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✦ Synopsis

Abstract

An enzymatic synthesis of [^11^C] N‐acetylserotonin is described. [1‐^11^C] acetate is produced by reacting ^11^CO~2~ with methylmagnesium bromide and converted to [^11^C]acetylCoA by passage over a first enzyme reactor containing immobilized acetylcoenzyme A synthetase. The produced [^11^C]‐acetylCoA is converted to [^11^C]N‐acetylserotonin with a second enzyme reactor containing immobilized acetylcoenzyme A: arylamine N‐acetyltransferase. The labelled end product is purified by means of ionexchange chromatography and is obtained in a solution suitable for intravenous injection.

After irradiation with 18 MeV protons at 15 μA for 20 min, 25 mCi with a specific activity of 250 mCi/μmol were obtained. The whole synthesis starting from EOB takes 40 min.

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