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Enzymatic synthesis of carbon-11 N-acetyl-D-glucosamine

✍ Scribed by Geert Mannens; Guido Slegers; Patrick Goethals; Albert Claeys

Publisher
John Wiley and Sons
Year
1990
Tongue
French
Weight
371 KB
Volume
28
Category
Article
ISSN
0022-2135

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✦ Synopsis

Abstract

An enzymatic synthesis of [^11^C]N‐acetyl‐D‐glucosamine is described. ^11^CO~2~ is reacted with methylmagnesium bromide to form [1‐^11^C]acetate. The latter is converted to [^11^C]acetylcoenzyme A by passage over an enzyme reactor containing immobilized acetylcoenzyme A synthetase (Acetate: CoA ligase (AMP forming); EC 6.2.1.1).

[^11^C]Acetylcoenzyme A is purified on‐line and concentrated on an anionexchange column. Elution is achieved with 1.5 M glucosamine HCl pH 8.0 and the [^11^C]acetylcoenzyme A fraction is collected in an ultrafiltration cell containing the enzyme glucosamine N‐acetyltransferase (acetylcoenzyme A: 2‐amino‐2‐deoxy‐D‐glucose N‐acetyltransferase EC 2.3.1.3).

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