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Automated synthesis of 11C-β-hydroxybutyrate by enzymatic conversion of 11C-acetoacetate using β-hydroxybutyrate dehydrogenase

✍ Scribed by Sébastien Tremblay; René Ouellet; François Bénard; Stephen C. Cunnane

Publisher
John Wiley and Sons
Year
2008
Tongue
French
Weight
113 KB
Volume
51
Category
Article
ISSN
0022-2135

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✦ Synopsis

Abstract

1‐[^11^C]‐β‐hydroxybutyrate was produced by conversion from 1‐[^11^C]‐acetoacetate using (D)‐β‐hydroxybutyrate dehydrogenase in the presence of nicotinamide adenine dinucleotide with purification by ion exchange column chromatography. Radiochemical yield at the end of the synthesis was 10% for a total synthesis time of 36 min. High‐performance liquid chromatography analysis showed ≤4% impurities, principally unconverted acetoacetate. Residual tetrahydrofuran (34±11 ppm) and ethanol (77±30 ppm) were well under the tolerable limits for human studies. Copyright © 2008 John Wiley & Sons, Ltd.

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