✦ LIBER ✦

Determination of the specific activity of NaB3H4

✍ Scribed by Martha H. Murtiashaw; Suzanne R. Thorpe; John W. Baynes

Publisher: Elsevier Science
Year: 1983
Tongue: English
Weight: 299 KB
Volume: 135
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(83)90709-1

No coin nor oath required. For personal study only.

✦ Synopsis

A method for the rapid determination of the specific activity of NaB3H4 is presented. NaB3H4 is used to reduce NAD+ to [3H]NADH, which is then isolated by anion exchange chromatography. The specific activity of the NaB3H4 is calculated from measurements of radioactivity and absorbance (340 nm) in the [3H]NADH fractions.

📜 SIMILAR VOLUMES

The synthesis of [3H]cadralazine of high

The synthesis of [3H]cadralazine of high specific activity

✍ Derek E. Brundish; Peter D. Kane 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 French ⚖ 350 KB

The title compound has been prepared by two different approaches. The first involved a five-stage "hot" synthesis.where the tritiation step was selective debromination of a cyclic Fbromo-a-&unsaturated ketone. double bond was protected by formal aromatisation. this intermediate was determined by pro

Radiosynthesis of tritium-labeled novel

Radiosynthesis of tritium-labeled novel nitromethylene neonicotinoids compounds with NaB3H4

✍ Chao Li; Xiao-Yong Xu; Ju-Ying Li; Qing-Fu Ye; Zhong Li 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 French ⚖ 111 KB

Paichongding and Cycloxaprid are two novel neonicotinoids with good industrialization prospects for their high activity against imidacloprid-resistant pest. In this paper, the radiosynthesis of [ 3 H 2 ]-Paichongding and [ 3 H 2 ]-Cycloxaprid was achieved using a NaB 3 H 4 reduction. The title compo

Purification of [3H]taurine of high spec

Purification of [3H]taurine of high specific activity

✍ Robert E. Hruska; Ryan J. Huxtable; Henry I. Yamamura 📂 Article 📅 1977 🏛 Elsevier Science 🌐 English ⚖ 216 KB

Synthesis and 3H NMR of 3H-alaproclate o

Synthesis and 3H NMR of 3H-alaproclate of high specific activity

✍ Stefan Bengtsson; Lars Gawell; Thomas Högberg; Christer Sahlberg 📂 Article 📅 1985 🏛 John Wiley and Sons 🌐 French ⚖ 310 KB

The antidepressant alaproclate (IJ i s a selective i n h i b i t o r o f neuronal serotonin reuptake w i t h 1 ow aff i n i t y f o r other receptor sites. (2, 2-( [ 2,5-3H2]-4-chl orophenyl)-2-methyl-2-propyl 2-aminopropanoate) with high specific a c t i v i t y (38 C i / m o l ) was prepared by h

Microscale synthesis of high specific ac

Microscale synthesis of high specific activity 3H-nitroglycerin

✍ J. J. Kawamoto; J. J. Brien; G. G. Marks; K. Nakatsu 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 French ⚖ 265 KB

A simplified method for chromatography o

A simplified method for chromatography of Dnp-leucine in the determination of intracellular specific activity of [3H]leucine

✍ Yoko Saito; James R. Florini 📂 Article 📅 1973 🏛 Elsevier Science 🌐 English ⚖ 188 KB

SHORT COMMUNICATIONS A Simplified Method for Chromatography of Dnp-Leucine in the Determination of Intracellular Specific Activity of [ 3H] Leucine Regier and Kafatos (1) have recently described a sensitive and convenient microtechnique for determination of the intracellular specific activity of [