The synthesis of multifunctional symmetric primary amines 4 and 8 and the covalent binding of 5'-0dimethoxytrityl-deoxynucleoside derivatives to their amino groups (compound 5 and 9) is described. Different strategies for dedimethoxytritylation including the use of strong acidic ion exchangers or pr
Criteria for the economic large scale solid-phase synthesis of oligonucleotides
β Scribed by F.Xavier Montserrat; Anna Grandas; Ramon Eritja; Enrique Pedroso
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- French
- Weight
- 442 KB
- Volume
- 50
- Category
- Article
- ISSN
- 0040-4020
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β¦ Synopsis
The experimental conditions necessary for reducing the cost of large-scale solid-phase oligonucleotide synthesis by the phosphite hiester approac41 have been studied using bigbly loaded polystyrene resins.
π SIMILAR VOLUMES
Nucleoside derivatives coupled to a multifunctional highly symmetric primary amine 3 built the fundamental of a convenient method for large scale oligonucleotide synthesis in solution. The basic purification for the fast isolation of intermediates is obtained by gel permeation chromatography. Monome
A convenient solid-phase synthesis of oligonucleotides conjugated at the 3Π-end with a tetraphenylporphyrin residue, by means of a new polymeric support bearing as a linker a lysine derivative, has been developed. A porphyrin linked