Photolabile linker for the solid-phase synthesis of base-sensitive oligonucleotides
β Scribed by Christelle Dell'Aquila; Jean-Louis Imbach; Bernard Rayner
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- French
- Weight
- 250 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0040-4039
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π SIMILAR VOLUMES
A photolabile linker based on a thiohydroxamic acid has been shown to be an efficient 'traceless' linker, revealing an aliphatic CH bond on photolysis at 350 nm. Methods for the synthesis, loading and photolytic cleavage of the linker are described.
The dithiane protected benzoin group is shown to be a useful safety catch photolabile linker in solid-phase synthesis. Methods for the synthesis, loading, deprotection and photolysis of the linker are described.
Linker phosphoramidite reagents containing a protected nucleoside with a cleavable 3%-ester linkage to either succinic acid, diglycolic acid, or hydroquinone-O,O%-diacetic acid (Q-Linker) allow the 3%-terminal nucleoside of an oligonucleotide sequence to be attached to underivatized 'Universal' amin