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Linker phosphoramidite reagents for oligonucleotide synthesis on underivatized solid-phase supports

โœ Scribed by Richard T Pon; Shuyuan Yu

Publisher
Elsevier Science
Year
2001
Tongue
French
Weight
124 KB
Volume
42
Category
Article
ISSN
0040-4039

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โœฆ Synopsis

Linker phosphoramidite reagents containing a protected nucleoside with a cleavable 3%-ester linkage to either succinic acid, diglycolic acid, or hydroquinone-O,O%-diacetic acid (Q-Linker) allow the 3%-terminal nucleoside of an oligonucleotide sequence to be attached to underivatized 'Universal' amino or hydroxyl supports. After solid-phase oligonucleotide synthesis, treatment with NH 4 OH cleaves the 3%-ester link to the support and produces oligonucleotides with only 3%-OH ends. No changes to existing phosphoramidite synthesis cycles, cleavage, or deprotection methods are required.

๐Ÿ“œ SIMILAR VOLUMES

Rapid automated derivatization of solid-
Rapid automated derivatization of solid-phase supports for oligonucleotide synthesis using uronium or phosphonium coupling reagents
โœ Richard T. Pon; Shuyuan Yu ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› Elsevier Science ๐ŸŒ French โš– 252 KB

Very fast (4 sec) attachment of nucleosides to CPG supports, via succinyl or QDA linkers, is possible with either uronium (HBTU, HATU, HBPyU, HBPipU) or phospbonium (BOP, PyBOP ยฎ, Py-BroP, BroP) coupling reagents and automated derivatization of CPG and polystyrene supports just prior to oligonucleot