✦ LIBER ✦

Rapid automated derivatization of solid-phase supports for oligonucleotide synthesis using uronium or phosphonium coupling reagents

✍ Scribed by Richard T. Pon; Shuyuan Yu

Publisher: Elsevier Science
Year: 1997
Tongue: French
Weight: 252 KB
Volume: 38
Category: Article
ISSN: 0040-4039
DOI: 10.1016/s0040-4039(97)00620-5

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✦ Synopsis

Very fast (4 sec) attachment of nucleosides to CPG supports, via succinyl or QDA linkers, is possible with either uronium (HBTU, HATU, HBPyU, HBPipU) or phospbonium (BOP, PyBOP ®, Py-BroP, BroP) coupling reagents and automated derivatization of CPG and polystyrene supports just prior to oligonucleotide synthesis has been demonstrated.