with K-selectride to afford the desired hydroxy compound 24. Desilylation of 24 with HF ' py gave the hydroxy oxime 25. The C=N bond of 25 was reduced with NaBH,CN providing the desired product 26 together with its C4 epimer.[131 Finally, chromatographically purified 26 was deprotected to the target
A Facile Solid-Phase Strategy for the Synthesis of Oligonucleotide-Tetraphenylporphyrin Conjugates
✍ Scribed by Lorenzo De Napoli; Stefania De Luca; Giovanni Di Fabio; Anna Messere; Daniela Montesarchio; Giancarlo Morelli; Gennaro Piccialli; Diego Tesauro
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 254 KB
- Volume
- 2000
- Category
- Article
- ISSN
- 1434-193X
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✦ Synopsis
A convenient solid-phase synthesis of oligonucleotides conjugated at the 3Ј-end with a tetraphenylporphyrin residue, by means of a new polymeric support bearing as a linker a lysine derivative, has been developed. A porphyrin linked
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An improved protocol for the synthesis of oligonucleotides synthesized and the corresponding triplexes have been analyzed by thermal denaturation experiments and CD containing a 3ЈϪ3Ј phosphodiester linkage, for use in an alternate strand triple-helix formation approach, is reported. spectroscopy. T