The use of liquid phase carriers for large scale oligonucleotide synthesis in solution via phosphoamidite chemistry

✍ Scribed by Ralf Wörl; Hubert Köster

• Publisher: Elsevier Science
• Year: 1999
• Tongue: French
• Weight: 821 KB
• Volume: 55
• Category: Article
• ISSN: 0040-4020
• DOI: 10.1016/s0040-4020(99)00059-9

✦ Synopsis

Nucleoside derivatives coupled to a multifunctional highly symmetric primary amine 3 built the fundamental of a convenient method for large scale oligonucleotide synthesis in solution. The basic purification for the fast isolation of intermediates is obtained by gel permeation chromatography. Monomer and dimer phosphoamidites (la-d; 2a, b) are used for the preparation of short oligodeoxynucleotides. Total cycle yields between 81 and 95 % and average cycle yields of 87 % were obtained. MALDI-TOF-mass spectrometry was used for the analysis of the fully protected intermediates during synthesis.