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Synthesis of new liquid phase carriers for use in large scale oligonucleotide synthesis in solution

✍ Scribed by Ralf Wörl; Hubert Köster

Publisher
Elsevier Science
Year
1999
Tongue
French
Weight
878 KB
Volume
55
Category
Article
ISSN
0040-4020

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✦ Synopsis

The synthesis of multifunctional symmetric primary amines 4 and 8 and the covalent binding of 5'-0dimethoxytrityl-deoxynucleoside derivatives to their amino groups (compound 5 and 9) is described. Different strategies for dedimethoxytritylation including the use of strong acidic ion exchangers or protic acids and modified silica gels and/or gel permeation chromatography are developed. The resulting liquid phase carriers 6 and 10 are suitable for large scale oligonucleotide synthesis in solution using phosphoamidites and gel permeation chromatography for fast isolation of intermediates.

📜 SIMILAR VOLUMES

The use of liquid phase carriers for lar
The use of liquid phase carriers for large scale oligonucleotide synthesis in solution via phosphoamidite chemistry
✍ Ralf Wörl; Hubert Köster 📂 Article 📅 1999 🏛 Elsevier Science 🌐 French ⚖ 821 KB

Nucleoside derivatives coupled to a multifunctional highly symmetric primary amine 3 built the fundamental of a convenient method for large scale oligonucleotide synthesis in solution. The basic purification for the fast isolation of intermediates is obtained by gel permeation chromatography. Monome