Comparison of electrochemiluminescence and amperometric detection of lipid hydroperoxides

✍ Scribed by S. Sakura; J. Terao

• Publisher: Elsevier Science
• Year: 1992
• Tongue: English
• Weight: 556 KB
• Volume: 262
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(92)80058-f

✦ Synopsis

Amperometnc measurement and electrochemdummescenee (ECL) were used for the detection of lipid hydroperonde m aqueous solutmns In the amperometnc measurements, bpld hydroperomde was detected by Its oxldatlon current at a glassy carbon electrode m a flow cell (EC0 method) This oxldatlon occurred at a very posltwe potential (1 10 V vs SCE) In the ECL method, lummol, which IS oxldlzed at a less positwe potential than lipid hydroperoxlde, was electrochemxxdly ondlzed to the excited intermediate (dnuaqumone) on the electrode, then the ondlzed lummol reacted with lipid hydroperomde which emltted bght The EC0 and ECL methods were compared at both glassy carbon and platinum electrodes m a flow-mjection system with phosphate buffer (PH 7 4)-30% acetomtrde tamer solution for measurement of hpld hydroperoxlde Uric actd, ascorbic acid and tocopherol, which are present m blood samples, Interfered wtth the ECL signal In the EC0 method, only unc acid and ascorbic acid interfered with the signal The detedon limit, relative standard devlatlon (R S D ) at the detectlon limit and signal-to-noise ratio (S/N) were as follows EC0 at a glassy carbon electrode, 0 05 nmol wth R SD 4 9% (n = 5) and S/N 136, ECL at a glassy carbon electrode, 0 3 nmol with R S D 13% (n = 5) and S/N 15, and ECL at a platinum electrode, 0 1 nmol with RSD 53% (n=S)and S/N 25