Analysis of lipid hydroperoxides
✍ Scribed by M. Carmen Dobarganes; José Velasco
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 40 KB
- Volume
- 104
- Category
- Article
- ISSN
- 1438-7697
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
The addition of luminol plus a catalyst such as peroxidase or a heme prosthetic group to a solution containing a small quantity of lipid hydroperoxides results in a flash of chemiluminescence, the intensity of which is a function of the hydroperoxide concentrations. Various protocols for lipid hydro
## Electrochenulummesnce (ECL) was used for the detectlon of the hpld hydropcronde methyl lmoleate hydroperoxlde (MLHP) at a glassy carbon electrode m acetomtnle (AN&neutral aqueous solution The cychc voltammograms of lummol and MLHP were measured to fmd the optimum apphed potential Lummol was oxl
Many assay techniques have been used to measure lipid hydroperoxides in plasma, including absorbance of conjugated dienes and reactivity with thiobarbituric acid. Because these measurements are not specific for lipid hydroperoxides, we modified an exisiting iodometric method to correct for interferi
Amperometnc measurement and electrochemdummescenee (ECL) were used for the detection of lipid hydroperonde m aqueous solutmns In the amperometnc measurements, bpld hydroperomde was detected by Its oxldatlon current at a glassy carbon electrode m a flow cell (EC0 method) This oxldatlon occurred at a
Pheophytin was heated with hydroperoxyoleate in the medium of methyl palmitate, methyl oleate or methyl linoleate to 60 "C in inert gas. The rate of pheophytin destruction due mostly to free peroxy radicals generated by hydroperoxide decomposition was proportional to the content of hydroperoxide. Do