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Amperometric Determination of Lipid Hydroperoxides

✍ Scribed by A. Mulchandani; D.C. Rudolph

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
444 KB
Volume
225
Category
Article
ISSN
0003-2697

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πŸ“œ SIMILAR VOLUMES

Comparison of electrochemiluminescence a
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Amperometnc measurement and electrochemdummescenee (ECL) were used for the detection of lipid hydroperonde m aqueous solutmns In the amperometnc measurements, bpld hydroperomde was detected by Its oxldatlon current at a glassy carbon electrode m a flow cell (EC0 method) This oxldatlon occurred at a

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## Electrochenulummesnce (ECL) was used for the detectlon of the hpld hydropcronde methyl lmoleate hydroperoxlde (MLHP) at a glassy carbon electrode m acetomtnle (AN&neutral aqueous solution The cychc voltammograms of lummol and MLHP were measured to fmd the optimum apphed potential Lummol was oxl

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The addition of luminol plus a catalyst such as peroxidase or a heme prosthetic group to a solution containing a small quantity of lipid hydroperoxides results in a flash of chemiluminescence, the intensity of which is a function of the hydroperoxide concentrations. Various protocols for lipid hydro

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✍ Gregory L. Cramer; James F. Miller Jr.; Robert B. Pendleton; William E.M. Lands πŸ“‚ Article πŸ“… 1991 πŸ› Elsevier Science 🌐 English βš– 916 KB

Many assay techniques have been used to measure lipid hydroperoxides in plasma, including absorbance of conjugated dienes and reactivity with thiobarbituric acid. Because these measurements are not specific for lipid hydroperoxides, we modified an exisiting iodometric method to correct for interferi