Determination of lipid hydroperoxides by electrochemiluminescence

✍ Scribed by S. Sakura; J. Terao

• Publisher: Elsevier Science
• Year: 1992
• Tongue: English
• Weight: 560 KB
• Volume: 262
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(92)80008-u

✦ Synopsis

Electrochenulummesnce

(ECL) was used for the detectlon of the hpld hydropcronde methyl lmoleate hydroperoxlde (MLHP) at a glassy carbon electrode m acetomtnle (AN&neutral aqueous solution The cychc voltammograms of lummol and MLHP were measured to fmd the optimum apphed potential Lummol was oxldlzed at about 0 45 V with or Hrlthout the presence of AN m the aqueous solution MLHP was oxldlzed at a fairly posItwe potential (1 10 V) The oxldatlon products were determined to be conjugated tnene, superoxlde and proton by consldermg UV spectral changes, colonmetric reactlons and reactlon with the superoxlde dlsmutase enzyme In the ECL method, lummol was oxldlzed on the electrode to the excited IntermedIate (dmzaqumone), whch reacted with MLHP and enutted hght ECL spectra and decay times were stuched In a flow-mjectlon system with pH 7 4 phosphate buffer-30% AN carrier solutlon, ermtted hght was detected at the oxldatlon potential of lunnnol The detectlon hnut was 0 3 nmol with a relative standard devlatlon of 13% (n = 5) at a agnal-to-nolsc ratio of 15