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Detection and characterization of lipid hydroperoxides at picomole levels by high-performance liquid chromatography

โœ Scribed by Yorihiro Yamamoto; Michael H. Brodsky; Jeffrey C. Baker; Bruce N. Ames

Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
523 KB
Volume
160
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A new method for the detection of various lipid hydroperoxides and hydrogen peroxide at the picomole level has been developed by combining an HPLC system with an ultrasensitive analytical system based on the detection of chemiluminescence emitted by isoluminol in the presence of hydroperoxide and microperoxidase. This HPLC separation removes interfering antioxidants so that the method can be applied to biological samples such as blood plasma lipids. Several HPLC conditions are described which allow simple identification of different lipid hydroperoxides.

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