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Separation of dimethylaminoazobenzenethiohydantoin amino acids by high-performance liquid chromatography at low picomole concentrations

✍ Scribed by Chao-Yuh Yang; Salih J. Wakil

Publisher: Elsevier Science
Year: 1984
Tongue: English
Weight: 307 KB
Volume: 137
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(84)90345-2

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✦ Synopsis

The separation of all common dimethylaminoazobenzenethiohydantoin (DABTH) amino acids derived from modified Edman sequencing can be achieved by using high-performance liquid chromatography. All derivatives, including DABTH-Ile and DABTH-Leu, can be readily separated in a solvent mixture of sodium acetate buffer and 1% ethylene dichloride in acetonitrile. The high absorbance of the DABTH amino acids at 436 nm makes possible the quantitative determination of these derivatives at picomole concentrations in a relatively short time (30-40 min).

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