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Rapid separation of amino acid phenylthiohydantoins by isocratic high-performance liquid chromatography

✍ Scribed by G.E. Tarr

Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
485 KB
Volume
111
Category
Article
ISSN
0003-2697

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✦ Synopsis

All common phenyhhiohydantoin amino acids derived from sequencing are identifiable in 6 to 9 min with an isocratic method based on buffered aqueous acetonitrile, an ODS stationary phase, and programmed flow which increases during the run. With constant flow the analysis requires 16 min. The effects of variation in percentage eluting solvent, ionic strength, anion, cation, pH, and temperature are defined.

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