All common phenyhhiohydantoin amino acids derived from sequencing are identifiable in 6 to 9 min with an isocratic method based on buffered aqueous acetonitrile, an ODS stationary phase, and programmed flow which increases during the run. With constant flow the analysis requires 16 min. The effects
Separation of amino acid phenylthiohydantoin derivatives by high-pressure liquid chromatography
β Scribed by Joseph L. Meuth; J.Lawrence Fox
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 486 KB
- Volume
- 154
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
separation of ethyl acetate-extractable phenylthiohydantoin-amino acids by high-pressure liquid chromatography with organic solvent gradients have been modified to facilitate sequence determination of proteins labeled with several radioactive amino acids at a time. ## Several laboratories have re
Phenylthiohydantoin (PTH) derivatives of all 20 common amino acids can be separated by high-pressure liquid chromatography. By using a Waters reversed-phase C18 column eluted with a concave ethanol gradient in ammonium acetate, pH 5.1, all PTH derivatives were eluted in less than 30 min. The NH,-ter
All the organic soluble amino acid phenylthiohydantoins are eluted (but not totally resolved) in under 40 min on either a Zorbax ODS or Permaphase ETH column. The superior resolution of the Zorbax ODS column and the complimentary characteristic of the Permaphase ETH column allow the isocratic analys