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High-pressure liquid chromatographic identification of phenylthiohydantoin derivatives of all twenty common amino acids

✍ Scribed by Jan Fohlman; Lars Rask; Per A. Peterson

Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
349 KB
Volume
106
Category
Article
ISSN
0003-2697

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✦ Synopsis

Phenylthiohydantoin (PTH) derivatives of all 20 common amino acids can be separated by high-pressure liquid chromatography. By using a Waters reversed-phase C18 column eluted with a concave ethanol gradient in ammonium acetate, pH 5.1, all PTH derivatives were eluted in less than 30 min. The NH,-terminal amino acid sequence of the human retinolbinding protein could unambiguously be established for the first 40 residues. Likewise, HLA-DR antigens biosynthetically labeled with [3H]tyrosine and [sH]phenylalanine were subjected to automatic sequential degradation. Labeled F'TH-amino acids were easily identified by the described chromatographic procedure.

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