𝔖 Bobbio Scriptorium
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A rapid method for the identification of amino acid phenylthiohydantoins by high performance liquid chromatography

✍ Scribed by Földi, J. ;Valentin, C. ;Cohen-Solal, M.

Publisher
John Wiley and Sons
Year
1980
Tongue
English
Weight
141 KB
Volume
3
Category
Article
ISSN
0935-6304

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✦ Synopsis

Thin-layer chromatogram (6 x 6 cm) of the model mixture: (1) inorganic phosphate, (2) choline, (3) choline phosphate, (4) glycerophosphorylcholine, (5) glycerophosphate, (6) betaine. Solvent systems: First direction (vertical), methanol/water/conc. NH4 OH/chlorotorm/acetone (5 : 2 : 1 : 2 : 2); Second direction (horizontal) methanol/water/conc. NH40H (5 : 3 : 1).

Visualization was rendered with iodine vapours (the second and the sixth spots) and malachite green reagent consecutively.

MS

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